Genes for PS II components

Complex Gene designation Gene product Amino acid residues 

Two genes (psbE and psbF) for polypeptides of Cyt b-559 have been,located and characterized from spinach [56], wheat [57], tobacco [58], Oenothera hookeri [58] and pea (D.L. Willey, unpublished). The gene for the 9 kDa polypeptide known to be associated with Cyt b-559 preparations was first of all located in spinach [59] and wheat [57] by hybrid-release translation or by coupled transcription-translation of fragments of chloroplast DNA. Nucleotide sequencing [56,57] revealed an open reading frame of 83 codons, encoding a protein with the known N- 

Translation products of nuclear genes for photosynthetic membrane components 

Several other open reading frames in the vicinity of these PS II genes have been located by nucleotide sequencing. Although the products of these open reading 

The genes for the hydrophilic polypeptides of the water-splitting complex have not been isolated, but their location in nuclear DNA is predicted from the synthesis of precursor forms of these polypeptides from poly(A) RNA isolated from spinach [65]. There is no evidence for the location of the genes for the other polypeptides associated with PS II. 

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