Generation of Disulfide-Containing Peptides

A disulfide-containing polypeptide chain needs to be cleaved into smaller fragments prior to mass spectrometry analysis. The cleavage of a protein generates two types of disulfide-containing peptides, intrachain (a or intramolecular) and interchain (b or intermolecular)  

TABLE 9.2. Reagents and Conditions for Cleaving Disulfide-Containing Proteins 

Cleaving Agent Digestion Conditions [Buffer, pH Temperature (°C)] Time (h) 

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