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General Strategies for Genetic Manipulation of Extreme Thermophiles

General Strategies for Genetic Manipulation of Extreme Thermophiles [Pg.513]

Organism Genetic modification Vector Transformation method Selection ) Counterselection ) Effective transformation efficiency ) (CFU/pgDNA) References VI 3 [Pg.514]

yayanosii Homologous recombination Plasmid CaCl2 + heat shock Sim (5 pM), -Ura 5FOA (0.1%) 10 [51] to S  [Pg.514]

onnurineus Homologous recombination Plasmid CaClj + heat shock Sim(lOpM) NA NR [54, 57] 2 a  [Pg.514]

barophilus Homologous recombination Plasmid Heat shock Sim (6 pM) 5FOA(46mM) 1Q2 [58, 59] S.  [Pg.514]



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Extreme

Extreme thermophile

Extremities

Extremizer

General strategy

Genetic manipulation

Thermophiles

Thermophiles extreme

Thermophilic

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