General Information about Layers and Solvents Used

General Information about Layers and Solvents Used 

Generally one of two principal adsorbents has been used for separating antibiotics with the help of TLC these are silica gel G (Firm 88), and, less often, alumina. Impregnated layers, e. g., with buffers, have occasionally been used. The solvents frequently used are compiled in Table 122. 

19 Ethyl acetate-di-n-butyl ether-water (upper layer) 

29 Butyl acetate-n-butanol-acetic acid-phosphate 

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