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Genes sequencing

Palmer, L. M., and Colwell, R. R. (1991). Detection of luciferase gene sequence in nonluminescent Vibrio cholerae by colony hybridization and polymerase chain reaction. Appl. Environ. Microbiol. 57 1286-1293. [Pg.426]

Goate, A.M., Cooper, D.N., Hall, C., Leung, T.K., Solomon, E., Li, L. (1987). Localization of a human heat shock hsp70 gene sequence to chromosome 6 and detection of two other loci by somatic cell hybrid and RFLP analysis. Hum. Gen. 75, 123-128. [Pg.454]

Table 1 Exemplary antiviral genes/sequences with proposed mode of action... Table 1 Exemplary antiviral genes/sequences with proposed mode of action...
Leigh Brown AJ (1997) Analysis of HIV-1 env gene sequences reveals evidence for alow effective number in the viral population. Proc Natl Acad Sd USA 94 1862-1865... [Pg.318]

Cluster Fx was also identified via its EPR spectral features in the RCI photosystem from green sulfur bacteria 31, 32) and the cluster binding motif was subsequently found in the gene sequence 34 ) of the (single) subunit of the homodimeric reaction center core (for a review, see 54, 55)). Whereas the same sequence motif is present in the RCI from heliobacteria (50), no EPR evidence for the presence of an iron-sulfur cluster related to Fx has been obtained. There are, however, indications from time-resolved optical spectroscopy for the involvement of an Fx-type center in electron transfer through the heliobacterial RC 56). [Pg.344]

Fig. 18 CG15920 gene sequence and primary structure. The consensus repeat sequences are also represented. The highlighted regions correspond to the signal sequence, R R chitin-binding domain, and the elastomeric domains containing repeat motifs A and B. Reproduced from [182, 188] with permission from Elsevier, copyright Elsevier 2001, 2010... Fig. 18 CG15920 gene sequence and primary structure. The consensus repeat sequences are also represented. The highlighted regions correspond to the signal sequence, R R chitin-binding domain, and the elastomeric domains containing repeat motifs A and B. Reproduced from [182, 188] with permission from Elsevier, copyright Elsevier 2001, 2010...
Figure 40-7. The polymerase chain reaction is used to amplify specific gene sequences. Double-stranded DNA is heated to separate it into individual strands. These bind two distinct primers that are directed at specific sequences on opposite strands and that define the segment to be amplified. DNA polymerase extends the primers in each direction and synthesizes two strands complementary to the original two. This cycle is repeated several times, giving an amplified product of defined length and sequence. Note that the two primers are present in excess. Figure 40-7. The polymerase chain reaction is used to amplify specific gene sequences. Double-stranded DNA is heated to separate it into individual strands. These bind two distinct primers that are directed at specific sequences on opposite strands and that define the segment to be amplified. DNA polymerase extends the primers in each direction and synthesizes two strands complementary to the original two. This cycle is repeated several times, giving an amplified product of defined length and sequence. Note that the two primers are present in excess.
Polymerase chain reaction (PCR) An enzymatic method for the repeated copying (and thus amph-fication) of the two strands of DNA that make up a particular gene sequence. [Pg.413]

Shine J., Eettes I., Lan N.C.Y., Roberts J.L. Baxter J.D. (1980) Expression of cloned j 8-endorphin gene sequences hy Escherichia coli. Nature, 285,456-461. [Pg.468]

It is obvious in situations like this that a clear phylogenetic picture is necessary in order to gain some understanding of the distributional history of the taxa in question, in this case the plicataloside-positive ones. This would appear to be a situation where gene sequence information might well provide the needed framework. It would also be of interest to learn where the biosynthesis of plicataloside diverges from the pathway that leads to the anthrones normally seen in Aloe. [Pg.10]

Plant phytoene synthase (Psy) has been used in a variety of transgenics. As noted above, P yl over-expression under a strong constitutive promoter caused a decrease in carotenoid accumulation, probably due to transcription silencing. Similarly, over-expression of the gene sequence backward (antisense) also silenced activity. In another approach to over-expression of tomato 1 in fruits, a synthetic alternative in which the third position of each codon was changed in order to avoid transcriptional silencing was successful in conditioning an increase in carotenoid accumulation. [Pg.376]

As already mentioned before a complete family of pectin lyases is present in A. niger. With all the genes sequenced and individually expressed, sometimes via promoter gene fusions (see below), the way is open to characterize the full spectrum of activities as outlined in the intro-... [Pg.333]

The -ATPase gene sequence indicates the presence of eight cysteine residues in the molecule [37,38]. In order to ascertain the chemical state of these cysteine residues, direct chemical studies with established cysteine and cystine reagents were carried out [44]. Titrations with the cysteine reagent, dithiobisnitrobenzoate, and the cystine reagent, nitrothiosulfobenzoate, indicated the presence of six free cy-... [Pg.122]

K6k M, R Oldenius, MPG van der Linden, P Raatjes, J Kingma, PH van Lelyveld, B Witholt (1989b) The Pseudomonas oleovorans alkane hydrolysis gene. Sequence and expression. J Biol Chem 264 5436-5442. [Pg.330]


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See also in sourсe #XX -- [ Pg.3 ]

See also in sourсe #XX -- [ Pg.3 ]




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Gene sequences

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