Gene expression nodule development

Sengupta-Gopalan, C. Pitas, J.W. (1986). Expression of nodule-specific glutamine synthetase genes during nodule development in soybeans. Plant Molecular Biology 7, 189-99. 

Besides the abundant nodulins like Lb, GS, uricase and sucrose synthetase, many other enzymes involved in nodule metabolism are induced during the course of nodule development (see Verma Nadler, 1984). However, owing to the relatively low abundance of these enzymes and their mRNAs in nodules, none of the cognate genes have been cloned and new cloning strategies need to be employed to isolate these relatively less abundantly expressed nodulin sequences. 

Konieczny, A., Szczyglowski, K., Boron, L., Przybylska, M. Legocki, A.B. (1988). Expression of lupin nodulin genes during root nodule development. Plant Science 55, 145-9. 

Scheres, B., van Engelen, F., van der Knaap, E., van de Wiel, C., van Kammen, A. Bisseling, T. (1990b). Sequential induction of nodulin gene expression in the developing pea nodule. The Plant Cell 2,687-700. 

Dickstein, R., Bisseling, T., Reinhold, V.N. Ausubel, F.M. (1988). Expression of nodule-specific genes in alfalfa root nodules blocked at an early stage of development. Genes and Development 2, 677-87. 

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