Gene expression cassette

Figure 15.2. Gene expression cassette. The drawing shows the features of the expression cassette. These inciude the promoter, 5 untransiated sequence, intron, Kozak sequence, therapeutic gene, 3 untrans-iated sequence, and the poiy-adenosine signai sequence.

Should the plasmid contain no CpG motifs, the introduction of some of these sequences outside of the functional sequences (i.e., origin of replication, antibiotic resistance, and relevant gene expression cassette) would be needed to render the plasmid functional for vaccination purposes. 

The eukaryotic expression cassette is the part of an expression vector that enables production of a protein in a eukaryotic cell. The cassette consists of a eukaryotic promoter for mRNA transcription, the gene and an mRNA termination and processing signal (Poly-A signal). 

B. Sjostrom et al. Correlation of gene expression of ten drug efflux proteins of the atp-binding cassette transporter family in normal human jejunum and in human intestinal epithelial caco-2 cell monolayers, /. Pharmacol. Exp. Ther. 2001, 299, 164-170... 

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