Gastric juice yeast

Martin et al. [22] treated Se-enriched yeast with simulated gastric juice containing 1 percent m/m pepsin (pH = 1.8, in 0.9 percent m/m NaCl) in a one-step procedure for 4 h. As expected, pepsin itself could provide partial proteolysis. Of the total Se content 11 percent could be identified as SeMet and Se(I V). 

The in vitro gastric and intestinal digests of selenized yeast food supplements were investigated by successive HPLC-ICP-MS and HPLC-ES-MS/MS [51]. The main compound extracted by both gastric and intestinal juice was Se-methionine, which was also the main Se compound extracted by proteolytic digestion from the yeast supplements. Two other minor compounds could be identibed as Se-cystine and Se(0)-methionine, a degradation product of Se-methionine [51]. 

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