Gastric Acid Secretion in Pylorus-Ligated Rats

Gastric Acid Secretion in Pylorus-Ligated Rats 

The secretory potential of a candidate compound might be much more problematic under safety aspects due to its ulcerogenic potential compared to the antisecretory potential of a candidate compound with a different primary indication. 

The volume of the collected gastric juice is measured. Acid concentration is measured by titration against 100 mm NaOH to an endpoint of pH 7. Total acid output (mmol H+/3 h) is calculated and percent inhibition of the treated rat group is calculated against the control group. Using various doses, dose-response curves can 

The pylorus-ligated rat has been proven to be a valuable method to evaluate the secretory (versus basal secretion) as well as the antisecretory potential (versus stimulation with histamine, gastrin, or carbachol) of a candidate compound with various secretory or antisecretory mechanisms of action. 

For the safety pharmacological evaluation this method allows the administration of necessary high doses by i.d. or i.p. administration probably as suspension (methylcellulose) irrespective of solubility issues of the candidate compound. 

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Barocelli, E., Ballabeni, V., Chiavarini, M., Impicciatore, M., 1995. R-a-methylhistamine-induced inhibition of gastric acid secretion in pylorus-ligated rats via central histamine H3 receptors. Br. J. Pharmacol. 115, 1326-1330. 

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