Gastric Acid Secretion in Anesthetized Stomach-Lumen Perfused Rats

Gastric Acid Secretion in Anesthetized Stomach-Lumen Perfused Rats 

Rats with a body weight of 300-350 g are used for measuring gastric acid secretion during anesthesia. The animals are fasted for 18 hours prior to the 

The perfusate is collected at 15 min periods and its acid concentration measured by titration against 100 mm NaOH to an endpoint of pH 7 and acid output ( i.mol H+/15 min) is calculated. Using various doses of the candidate compound and of a standard, dose-response curves can be established and activity ratios with confidence limits can be calculated. 

For the specific pharmacological assessment of inhibitors of gastric acid secretion, like H2-blockers, anticholinergics, H+/K+-ATPase inhibitors, this method reveals valid results with respect to the antisecretory potential of the candidate compound. Limitations of this methods with respect to safety pharmacological assessment of candidate compounds are (1) only parenteral administration of the candidate compound, preferentially i.v., is feasible and should be preferred, and (2) only antisecretory putative side effects can be investigated this method is of limited relevance to study secretory side effect potential of candidate compounds. 

For safety pharmacological assessment of a candidate compound with a totally different inherent primary indication, its antisecretory potential at supra-pharmacological doses has to be studied. Therefore, whether this method can be used for the safety evaluation depends on the solubility of 

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