Gas chromatograph compounds

An index characteristic of a gas chromatographed compound on a given column at a definite temperature defined as ... 

Hengen and Hengen developed a method for the determination of nicotine and cotinlne in 1 ml samples of plasma. Nicotine was extracted from alkalinized plasma (NaOH) with diethyl ether, and cotinlne from the same sample with dichlbromethane. Modaline was used as internal standard for nicotine, lidocaine for cotinine. Analytical recovery of nicotine added to the plasma was 80 - 6 %, for cotinine 95 - 5 56. The internal standards were directly added to the plasma to monitor extraction losses. The sensitivity was such that less than 0.1 pg of nicotine and 0.1 pg of cotinine could be detected per liter. Day-to-day reproducibility for nicotine was within 14 % and within 6 % for cotinine. Narrow peaks for the gas chromatographed compounds were obtained on the very stable SP-2250 column (3 %) on Supelcoport at 155°C for... 

Chemical Analysis. The quantity of gas chromatographable organic compounds were determined as described previously (4, 5). For GC/MS analysis, a Finnigan model 4023 gas chromato-grapH/mass spectrometer and I NCOS data system were used to obtain 70 ev electron impact mass spectra of gas chromatographable compounds. A 25 m OV-101 quartz capillary column was used. Carrier gas was helium with a flow velocity of 30 cm/sec. The temperature program was 50 C for two minutes and then 5 per minute to 270 . The final temperature was held for 15 minutes. Samples were injected (splitless injection) in 1 uL of dichloromethane at a concentration of 2 yg per yL. 

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