Galacto Galactosidase

P 19] Two micro syringes were filled with 0.32 mM p-nitrophenyl-y9-D-galacto-pyranoside in phosphate buffer (pH 8) and /3-galactosidase (20 U) in 10 ml of the same buffer [26]. Both solutions were pumped into the micro channel at the same flow rate (a few pi min ). The reaction was carried out for 0-30 min at 37 °C using a hot-plate. The residence time was set by adjusting the flow rate. After passing the micro channel, the reaction mixture was dropped into hot water to inactivate the enzyme. 

J-Galactosidase Galacto-oligosaccharides 7000-15,000 Yacult (Japan), Milupa/Numico (Germany)... 

Lane I, standard brain gangliosides. Lane 7, standard neutral glycolipids from bovine erythrocytes. Lane 2, ganglioside II. Lane 3, 2+ neuraminidase. Lane 4, i+ fi-galacto-sidase. Lane 5, 4+ /3-hexosaminidase. Lane 6,5+fi-galactosidase. 

Samples Prepare a solution of a-galactosidase sample in Acetate Buffer that contains between 0.008 and 0.024 galactos-idase units of activity per milliliter. 

Based on the K values for methyl-/3,D-galacto-pyranoside and methyl-/3,D-thiogalactoside, which compound is the more potent inhibitor of /3-galactosidase ... 

Lee S, Kim B. trans-Sialidase catalyzed sialylation of P-galacto- 29. syldisaccharide with an introduction of P-galactosidase. Enzyme Microb. Technol. 2001 28 161-167. 

Fig. 21 Evidence of successful gene transfection with bacterial P-galactosidase vectors with IL in hypoxic cells, or with cationic liposomes or IgG-liposomes. The P-galactosidase activity was developed with X-Gal at pH 7.0. Cells treated with IL-P-galacto-sidase vector showed many cells with blue color. The culture was also confluent. Cells treated with cationic liposome-P-galactosidase vector showed only two cells with gene expression. The cells were also not confluent indicating cell attrition. IgG-liposome treated cells also showed no P-galactosidase expression.

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