Ribonuclease fungal

Various bacterial ribonucleases as well as the fungal ribonucleases Ty Uj, and U2 (see also Fig. 5-43) have amino acid sequences related to that of RNase A763 764 764a but with distinctly different three-dimensional structures. The active sites contain Glu, His, and Arg side chains. For RNase Ty Glu 58 and His 92 appear to provide acid-base catalysis with assistance from Tyr 38, Arg 77, and His 40.763 765 A glutamate carboxylate also appears to be the catalytic base in the related RNase, called barnase, from Bacillus amyloliquefaciens.766... 

Riboflavin 5 -phosphate (flavin mononucleotide, FMN) 513, 765,780, 781s Ribonuclease 264, 647,648 fungal 648... 

Kao R, Davies J Molecular dissection of mitogillin reveals that the fungal ribotoxins are a family of natural genetically engineered ribonucleases. J Biol Chem 1999 274 12576-12582. 

Protoplasts, produced by enzymatic hydrolysis of the cell walls, have been used as a source of plant organelles and could be used as a source of soluble enzymes, but the methods can only be applied to relatively soft tissues and require prolonged exposure of tissues to cell wall degrading enzymes >. In addition, most of the fungal enzyme preparations that ea e used contain such undesirable products as proteases, ribonucleases, peroxidases and phenolics. ... 

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