Fructan 1-Exohydrolase

Biology and Chemistry of Jerusalem Artichoke Helianthus tuberosus L. 

FIGURE 10.20 The action of fructan 1-exohydrolase and fructan fructan 1-fructosyltransferase in dormant and sprouting tubers on inulin depolymerization. Abbreviations 1-feh = fructan 1-exohydrolase 1-fft = fructan fructan 1-fructosyltransferase hk = hexokinase spp = sucrose phosphate phosphatase sps = sucrose phosphate synthase GF = sucrose G = glucose F = fructose GFF = 1-ketose GF-P = sucrose phosphate UDP-G = uridine diphosphate glucose F-6-P = fructose-6-phosphate. 

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Dideoxy-2,5-imino-D-mannitol 7 (Scheme 3) in complex (PDB 2AEY) with chicory fructan 1-exohydrolase (EC 3.2.1.153 GH 32) was found to bind in a similar way as does fructose, as well as the fructofuranosyl subunit of sucrose and... 

M. Verhaest, W. Lammens, K. Le Roy, C. J. De Ranter, A. Van Laere, A. Rabijns, and W. Van den Ende, Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase 1-Kestose as substrate vs sucrose as inhibitor, New Phytol., 174 (2007) 90-100. 

Van den Ende, W., Michiels, A., De Roover, J., Verhaert, P., and Van Laere, A., Cloning and functional analysis of chicory root fructan 1-exohydrolase I (1-FEH I) a vacuolar enzyme derived from a cell-wall invertase ancestor Mass fingerprinting of the 1-FEH I enzyme, Plant J., 24, 447 156, 2000b. 

Henson, C.A. and Livingston, D.P., III, Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyses -2,6-fructans, Plant Physiol., 110, 639-644, 1996. 

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