Frontal affinity chromatography FAC

One of techniques is frontal affinity chromatography (FAC) coupled to MS [109]. In this method, a column is prepared with an immobilized biological... 

An alternative approach for equilibrium constant measurements in affinity chromatography is to use frontal analysis. This method is sometimes known as frontal affinity chromatography FAC). In this technique, a solution containing a known concentration of the analyte is continuously applied to an affinity column at a fixed flow rate (Fig. 2). As the analyte binds to the immobilized ligand, the ligand... 

Slon-Usakiewicz, J.J. Ng, W. Dai, J.-R. Pasternak, A. Redden, P.R. Frontal affinity chromatography with MS detection (FAC-MS) in drug discovery. Drug Diseov. Today 2005,... 

K. Kelly, M. Frontal Affinity Chromatography Mass Spectrometry (FAC-MS) Method Evaluation with a Nuclear Hormone Receptor, in Proceedings of the 49th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 11-15, 2000, TPF 228. 

Automated multiple ligand screening by frontal affinity chromatography-mass spectrometry (FAC-MS). J. Biomol. Screen., 12, 167-174. 

Frontal affinity chromatography-mass spectrometry (FAC-MS) is very popular for ligand and inhibitor discovery and design [36], This two-dimensional detection method physically separates compounds by affinity chromatography and determines their identities by MS. Introduced in 1999 [37], FAC-MS is capable of ranking several compounds present in solution in terms of their binding... 

Ng,W., Dai, J. R., Slon-Usakiewicz, J. J, Redden, P. R., Pasternak, A., Reid, N. (2007). Automated multiple ligand screening by frontal affinity chromatography-mass spectrometry (FAC-MS). J. BiomoL Screen., 12, 167-174. 

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