Fragment screening biochemical assays

J. Barker, T. Hesterkamp, M. Schade and M. Whittaker, Fragment screening Biochemical assays versus NMR, Innov. Pharm. Tech., 23, 19-22 (2007). 

Barker, J., Courtney, S., Hesterkamp, T., Ull-mann, D., Whittaker, M. (2005) Fragment screening by biochemical assay. Exp Opin Drug Discov 1, 225-236. 

Protein targets, which are even too costly for the 5000 subset, can be prescreened by virtual screening (VS) or a biochemical assay and the NMR assay restricted to an economical library size of 100-1000 hit compounds. Taken together, we believe that it pays off to screen experimentally as many fragments as economical for a target. This notion appears to have been adopted by other proponents in NMR screening, such as Abbott with an NMR library of approximately 10 000 compounds (Table 3.2). 

J. Barker, S. Courtenay, T. Hesterkamp, D. Ullmann and M. Whittaker, Fragment screening by biochemical assay, Expert Opin. Drug Discov., 1, 225-236 (2006). 

Mikuni J et al (2010) A fluorescence correlation spectroscopy-based assay for fragment screening of slowly inhibiting protein-peptide interaction inhibitors. Anal Biochem 402(1) 26-31... 

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