Formaldehyde release enzymatic methods

Enzymatic Methods for Determining Formaldehyde Release from Wood Products... 

Two sensitive fluorometric enzymatic methods for the determination of formaldehyde release from wood products were described. These methods were developed using the enzyme formaldehyde dehydrogenase to catalyze the oxidation of formaldehyde to form formic acid and NADH in the presenc of oxidized nicotinamide adenine dinucleotide (NAD ). The increase in NADH, which is directly proportional to the concentration of formaldehyde, is measured fluorometrically at em ... 

We have developed two novel new enzymatic fluorometric methods for the trace analysis of formaldehyde. Due to their simplicity, sensitivity and specificity, these methods should find wide applications in the monitoring of formaldehyde released from wood products. As we stated above, enzymatic fluorometric method II does offer higher sensitivity and better detection limit over enzymatic fluorometric method I. However, method II requires two... 

Several methods have been developed for assaying non-enzymatic glycosylation. As far as biological systems are concerned, these have been extensively reviewed by A. J. Furth in 1988 (5). They include both assays on intact proteins after chemical degradation and selective detection of e.g. 5-hydroxymethylfurfural (HMF) and formaldehyde using the thiobarbituric assay (TEA), and assays on protein hydrolysates with or without previous reduction of the protein-bound Amadori compound. In this last case, the analysis is based on the determination of furosine which is specifically formed from lysine Amadori compounds with a yield of approximately 30% (6). The furosine method, originally developed for milk (7), has been the subject of several analytical improvements both for food products (8) and biological materials (9). More recently, another method has been proposed to evaluate the extent of early Maillard reaction in milk products. This method is based on direct measurement of the Amadori product lactuloselysine which is released after complete enzymatic hydrolysis (10). 

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