Folate human subject

A study of 2K human subjects revealed that treatment with three vitamins together (folate, vitamin and vitamin Bu) can provoke a decline of plasma homt>cysteine from an initial level of about 12 piM to the lower level of 8 xM (Naurath ei a]., 1995). A study of 100 men with moderate levels of plasma homocysteine (18-40 pM) involved separate supplements of placebo, folic acid, vitamin 15i2, or vitamin E. Folic add alone resulted in a 40% decline in plasma homocysteine. Vitamin B] alone provoked a 15% decrease in the amino acid, while vitamin... 

The effect of antimicrobial agents on serum folate level was studied using the standard autoclaving technique of Lactobacillus casei microbial assay. The blood for serum folate assay was obtained from adult human subjects before and 2 hours after a 24 hours test dose of the antibiotic (72). The results show that lincomycin produced a falsely low serum folate level. 

Krumdieck CL, Fukushima K, Fukushima T, Shiota T, Butterworth CE, Jr. A long-term study of the excretion of folate and pterins in a human subject after ingestion of 14C folic acid, with observations on the effect of diphenyUiydantoin administration. Am J Clin Nutr 1978 31 88-93. 

Many short-term kinetic studies have been reported on the basis of plasma and senun folate levels in humans. Typical protocols involve the administration of a folate tracer dose to folate-saturated subjects or else a large folate dose given to subjects of more normal folate status (Reich and Gonczy, 1979 Bunni et aL, 1989 Wolfrom et al, 1990 Priest et al, 1991 von der Porten et al, 1992). Although these studies have clinical relevance with respect to the short-term kinetics of acute doses of the vitamin (e.g., postchemotherapy 5-formyl-tetrahydrofolate), they often provide little or no information about the major body folate pool that exhibits much slower kinetics. 

Gregory, J. F., Bailey, L. B., Thomas, E. A., Toth, J. P., Cerda, J. J., and Fisher, W.R. (1994). Stable-isoto M study of long-term folate metabolism in a human subject. FASEB J. 8, A920. 

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