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Fluram, fluorescamine

Fluorescamine (Fluram). Fluorescamine was developed from the study of ninhydrin chemistry as a reagent for detecting amino groups (Udenfriend et al., 1972) and has been successfully used in the reversed phase chromatographic analysis of proteins and peptides using an on-line post-column detection system. This reagent requires two separate pumps for the delivery of the mobile phase buffer and the reagent and this method may therefore be considered to be less... [Pg.187]

Fig.4.41. The reaction of fluorescamine (Fluram ) with primary amines 1 = reagent II - amine III = fluorescent product. Fig.4.41. The reaction of fluorescamine (Fluram ) with primary amines 1 = reagent II - amine III = fluorescent product.
Air drawn through a glass tube containing ion exchange resin XAD-2 MIC desorbed from the resin into a solution of fluorescamine (Fluram) in tetrahy-drofuran an intense fluorescent derivative formed derivative analyzed by HPLC using a multiwavelength fluorescent detector (Vincent and Ketcham, 1980) recommended air flow rate 200 mL/min sample volume 15 L. [Pg.358]

Fluorescamine is 4-phenylspiro[furan-2(3H),1-phthalan] 3,3 dione. MIC should react with fluram according to the following equation ... [Pg.125]

Fluorometric detection. Greater specificity in detection may be obtained by derivatization and this has been used in conjunction with Ruo-rometric detection. The two principal derivatization reagents employed have been fluorescamine (Fluram—trademark of Hoffmann-La Roche Inc.) (F4, F6) and o-phthalaldehyde (B3, C9). The latter reagent gives a higher quantum yield, is soluble in aqueous buffers, and is 5-10 times as sensitive as fluorescamine (B3, C9). Both fluorescamine... [Pg.262]

Two new chromophoric reagents, 2-methoxy-2,4-diphenyl-3(2/f)-furanone (MDPF) (49) and 4-phenylspiro[furan-2(3H)-r-phthalan]-3,3 -dione (fluorescamine, trade name Fluram) (50) were introduced for the correlation of... [Pg.125]

Figure 1. Fluorogenic reactions of fluorescamine (Fluram) and MDPF with primary... Figure 1. Fluorogenic reactions of fluorescamine (Fluram) and MDPF with primary...
See other pages where Fluram, fluorescamine is mentioned: [Pg.535]    [Pg.485]    [Pg.485]    [Pg.535]    [Pg.392]    [Pg.441]    [Pg.441]    [Pg.535]    [Pg.485]    [Pg.485]    [Pg.535]    [Pg.392]    [Pg.441]    [Pg.441]    [Pg.294]    [Pg.156]    [Pg.467]    [Pg.484]    [Pg.484]    [Pg.124]    [Pg.129]    [Pg.144]    [Pg.145]    [Pg.191]    [Pg.156]    [Pg.121]    [Pg.185]    [Pg.187]   
See also in sourсe #XX -- [ Pg.535 ]

See also in sourсe #XX -- [ Pg.392 ]



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Fluorescamine

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