Fluorescent staining derivatives

Fig. 2 Left cytoplasmic microtubules in interphase kidney epithelial cells imaged with the fluorescent paclitaxel derivative Flutax-2 (green) and nuclear DNA stained with Hoescht 33342 (blue). Right mitotic spindle from a dividing metaphase cell with similarly imaged microtubules and chromosomes. Bars indicate 10 pm (micrographs courtesy of Isabel Barasoain)...

A EXPERIMENTAL FIGURE 10-5 Simple-sequence DNAs are useful chromosomal markers. Human metaphase chromosomes stained with a fluorescent dye were hybridized in situ with a particuiar simpie-sequence DNA iabeied with a fluorescent biotin derivative. When viewed under the appropriate wavelength of light, the DNA appears red and the hybridized simple-sequence DNA appears as a yellow band on chromosome 16, thus locating this particular simple sequence to one site in the genome. [See R. K. Moyzis et at, 1987, Chromosoma 95 378 courtesy of R. K. Moyzis.]... 

Mao, R Leung, W. Haugland, R. R Sulfonated xanthene derivatives synthesis and applications as fluorescent stains. PCX Int. Appl. WO 9915517, 1999 Chem. Abstr. 1999,130, 264438... 

Aminoglycoside antibiotics and / -substituted indoles are stained red. Pyrrole derivatives with free / -positions react at room temperature to yield blue-colored zones [11]. Exposure to the vapors of aqua regia deepens the colors. This reaction sometimes produces fluorescence [3]. The detection limit for monomethylhydrazine is 200 pg per chromatogram zone [3]. 

In order to derive a quantitative relation between emission Intensity as measured by EMI and actual metal content, cell samples were subjected to graphite furnace atomic absorption (GFAA) analysis (14). Atomic absorption experiments were performed both on cells which had been stained with a fluorescent reagent and on cells not exposed to a lumlnophore. After EMI analysis, 50 fiL of cell suspension were withdrawn from the 0.30 mL of sample used for EMI and were digested In 150 iiL of concentrated HNO3 for 90 minutes at 85° . These solutions were then diluted to 1/10 of their concentration with deionized water, and the 150 liL of these diluted... 

The identification of anionic polyfacrylic acid) sizes can be carried out by staining with a fluorescent cationic dye (Cl Basic Orange 14) followed by spectroscopic measurement of excitation wavelength and fluorescence emission [195,196]. Such methods can also be used (with Cl Basic Orange 14 or Cl Basic Red 1) to detect and estimate carboxymethylcellulose, poly(vinyl alcohol) and starch derivatives [197]. 

Texas Red hydrazide is a derivative of Texas Red sulfonyl chloride made by reaction with hydrazine (Invitrogen). The result is a sulfonyl hydrazine group on the No. 5 carbon position of the lower-ring structure of sulforhodamine 101. The intense Texas Red fluorophore has a QY that is inherently higher than either the tetramethylrhodamine or Lissamine rhodamine B derivatives of the basic rhodamine molecule. Texas Red s luminescence is shifted maximally into the red region of the spectrum, and its emission peak only minimally overlaps with that of fluorescein. This makes derivatives of this fluorescent probe among the best choices of labels for use in double-staining techniques. 

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