Fluorescent protein conjugate

Steiner, R. F., and H. Edelhoch Fluorescent protein conjugates. Chem. 

Dandliker, W. B., Portmann, A. J. Fluorescent Protein Conjugates. In Excited States of Proteins and Nucleic Acids. Steiner, R. F., Weinigh, I. (Eds.) p. 199, New York Plenum 1971... 

Barak LS, Ferguson SSG, Zhang J, Martenson C, Meyer T, Caron MG. Internal trafficking and surface mobility of a functionally intact P2-adrenergic receptor-green fluorescent protein conjugate. Mol Pharmacol 1997 51 177-184. 

AR is the maximum standard intensity change of the fluorescence emission between saturated and unsaturated kinase. It is another important criteria for characterizing a fluorescent protein conjugate as suitable for sensitive fluorescence spectroscopic detection of conformational changes (10). Ideally, the sliould have a value >1.25 (although lower values are also acceptable) and is calculated using the equation ... 

Feng, X, Zhang, J, Barak, LS, Meyer, T, Caron, MG and Hannun, YA (1998) Visuahzation of dynamic trafficking of a protein kinase C betall/green fluorescent protein conjugate reveals differences in G protein-coupled receptor activation and desensitization. Journal of Biological Chemistry, 273, 10755-10762. 

In a fluorescent protein conjugate that behaves in solution as a rigid rotator Eq. (32) shows that two of the parameters that determine the observed polarization, namely ro and po, depend on the attached molecule while p, the relaxation time of the rotation, depends mainly on the macromolecule. It is well to inquire what changes in these quantities are introduced by the conjugation. 

Hodgson, L. Qiu, W Dong, C. Henderson, A. J. Use of green fluorescent protein-conjugated P-aactin as a novel molecular marker for in vitro tumor ceU chemotaxis assay. Biotechnol. Prog. 2000, 16, 1106-1114. 

Smith, S. N. and Steer, R. P. (2001). The photophysics of Lissamine rhodamine-B sulphonyl chloride in aqueous solution implications for fluorescent protein-dye conjugates. J. Photochem. Photobiol. A Chem. 139, 151-156. 

The bilin content of these fluorescent proteins ranges from a low of 4 prosthetic groups in C-phycocyanin to the 34 groups of B- and R-phycoerythrin. Phycoerythrin derivatives, therefore, can be used to create the most intensely fluorescent probes possible using these proteins. (Strept)avidin-phycoerythrin conjugates, for example, have been used to detect as little as 100 biotinylated antibodies bound to receptor proteins per cell (Zola et al., 1990). 

Keywords Conjugated polyelectrolyte DNA Energy transfer Fluorescence Protein Sensor... 

Table 14.2. Fluorescence Lifetime Distributions of Some Fluorophore-Protein Conjugates...

Giloh, H. and Sedat, M. (1982) Fluorescence microscopy reduced photobleaching of rhodamine and fluorescein protein conjugates by A-propyl gallate. Science 217, 1252-1255. 

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