Fluorescent complexes template

Formation of fluorescent complex upon binding of metal ion template... 

In an approach with an external modifier that increases the fluorescence of the polymer, an external H-labeled template was used to generate the fluorescent signal through proximity scintillation [51]. The difference in this case is that the binding of H-labeled template gives a fluorescent complex, and its displacement by the analyte results in a decrease in fluorescence. Therefore, a scintillation monomer, 4-hydroxymethyl-2,5-diphenyloxazole acrylate (Fig. 9, 40), was incorporated into the imprinted polymer. Since (3 particles travel only a short distance, the 3 emission of... 

Macrocyclic complexes of zinc have inspired interest in varied areas such as supramolecular and biomimetic chemistry including hydrolysis enzymes, such as phosphatases and esterases, and also for the fluorescent detection of zinc. The polyaza macrocycles and their A--functionalized derivatives are particularly well represented. An important aspect of macrocycle synthesis is the use of metal templates to form the ligand. Examples of zinc as a template ion will be discussed where relevant. 

Rothwell, P. J., Berger, S., Kensch, O., Felekyan, S., Antonik, M., Wohrl, B. M., Resde, T., Goody, R. S., and Seidel, C. A. (2003). Multiparameter single-molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptaseiprimer/template complexes. Proc. Natl. Acad. Sci. USA 100, 1655-1660. 

The interaction of artificial metal ions/complexes with peptides/proteins [11], nucleic acids/DNA [12,13], enzymes [14], steroids [15] and carbohydrates [16] forms a bridge between natural and artificial macromolecular metal complexes. Biometal-organie chemistry concentrates on such complexes [17]. The reason for the increasing interest in this field lies in medical applications of metal complexes (cancer, photodynamic therapy of cancer, immunoassays, fluorescence markers, enantioselective catalysis, template orientated synthesis of peptides, etc.). Figure 2-4 presents an overview of metals in medicine [18]. Some examples are given below. 

A primary indication on how well the monomers have been chosen is to simply see whether they are capable of assisting solubilization of the template in the prepolymerization mixture. A small-scale solubility test may thus be a good way to initially screen the monomers for strong monomer-template interactions. Weak interactions may be revealed by complexation induced spectral changes (in NMR,UVor fluorescence spectra). The complexation induced shifts of the characteristic H-NMR signals of the template upon increasing monomer concentrations are often used to estimate the monomer-template association constants. Prior to this, however, knowledge about the stoichiometry of the monomer-template complexation and the tendency of the monomer and template to self-associate are required.The former can be obtained by means of a so-called Job s plot whereas the latter by a dilution experiment. 

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