Fluorescent bead, time-resolved fluorescence

C. Application 1 Femtosecond Time-Resolved Fluorescence erom a Fluorescent Bead... 

In the first example, we describe time-resolved fluorescence measurements of a fluorescent bead (Fujino and Tahara 2004). Figure 3.6a shows the CCD image of a commercial fluorescent bead that has a diameter of -4.85 pm (Mag Sphere). This bead was laser trapped near the focus point by the excitation pulse. In fact, when the irradiation... 

FIGURE 3.6 The CCD image of a fluorescent bead under (a) laser trapping, and (b) without laser trapping, (c) The femtosecond time-resolved fluorescence at 520 nm observed with lOOx objective lens. (Form, Fujino, T. and Tahara, T.,Appl. Phys. B 79 145-151, 2004.)... 

The combination of different fluorescent metal indicators with inert luminescent reference beads consisting of poly(acrylonitrile) containing Ru(dpp)3 leads to a sensor array in a microwell plate format, suited for ratiomet-ric time-resolved imaging [95]. The data can be acquired with the help of the f-DLR method (for details see Sect. 2.3). A cross-reactive sensor array was arranged for the determination of mixtures of calcium(II), copper(II), nickel(II), cadmium(II), and zinc(II) ions by nine different commercially available fluorescent indicators (Table 3). For a successful application, it is mandatory that all luminophores can be excited at the same wavelength range between 400 and 500 nm, and that the excitation and emission spectra of all indicators overlap with those of the reference dye encapsulated in the nanobeads. 

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