Fluorescence spot test

Detection of glucose-6-phosphate dehydrogenase deficiency in erythrocytes a spectrophotometiic assay and a fluorescent spot test compared with a cytochemical method. 

Methods for evaluating the status of enzymes in the glycolytic cycle in red cell hemolysates have been described using simple fluorescence spot tests which allow detection of enzyme defects in a group of enzymes, with determination of the speciflc defect deferred until later and applied only to those individuals found to be abnormal by the screening methods employed (Yunis, 1973). 

Qualitative. The classic method for the quaUtative determination of silver ia solution is precipitation as silver chloride with dilute nitric acid and chloride ion. The silver chloride can be differentiated from lead or mercurous chlorides, which also may precipitate, by the fact that lead chloride is soluble ia hot water but not ia ammonium hydroxide, whereas mercurous chloride turns black ia ammonium hydroxide. Silver chloride dissolves ia ammonium hydroxide because of the formation of soluble silver—ammonia complexes. A number of selective spot tests (24) iaclude reactions with /)-dimethy1amino-henz1idenerhodanine, ceric ammonium nitrate, or bromopyrogaHol red [16574-43-9]. Silver is detected by x-ray fluorescence and arc-emission spectrometry. Two sensitive arc-emission lines for silver occur at 328.1 and 338.3 nm. 

Modern Techinques in Electroanalysis. Edited by Petr Vanysek Total Reflection X-Ray Fluorescence Analysis. By Reinhold Klockenkamper Spot Test Analysis Clinical, Enviromental, Forensic, and Geochemical Applications, Second Edition. By Ervin Jungreis... 

Field identification. Supplementary chemistry verification by x-ray fluorescence spectroscopy or chemical spot test (e.g., moly for 316 stainless steel) may be required. 

Field Identification. Supplemental chemistry verification of valve body by x-ray fluorescence spectroscopy or chemical spot test (e.g., for moly in 316 stainless steel) may be required. The stem is usually too narrow to be identified by x-ray fluorescence spectroscopy and supplementary means of analysis may be required. 

It would have taken roughly three weeks to complete the identification with an x-ray fluorescence analyzer, but with the use of a simple chemical spot test the job was completed in significantly less time. 

Lettucenin A (153) showed a greenish yellow fluorescent spot of Rf (diethyl ether, silica gel) 0.16 under u.v light (365 nm). It completely inhibited spore germination of Ceratocystis fimbriatata at concentrations of 2 pg/ml. Its antifungal activity was tested against Bipolaris leersiae. 

Inorganic species were characterised by means of atomic absorption, ion chromatography. X-ray fluorescence. X-ray diffraction as well as spot-test analysis [15] and mass balance. The catalyst was also characterised by means of BET surface area and pore volume. 

To label cells, test labeling with 1-100 ng/mL of the antibody for 10 min at room temperature. For subsequent experiments, choose antibody concentration that produces clearly separated fluorescence spots in single-molecule imaging. 

Methods Natural and Artificial Radioactivity Radionuclide Monitoring Radiotracers. Spot Tests. Thin-Layer Chromatography Overview. Titrimetry Oven/iew Potentiometric. X-Ray Fluorescence and Emission X-Ray Fluorescence Theory. 

Pontachrome Blue-Black R (zinc salt of 4-sulfo-2,2 -dihydroxyazo-naphthalene, C. I. Mordant Black 12) gives an orange-red fluorescence with aluminum salts in acetic acid solution Idn. Limit 2 y A1 in 1 ml). This test appears to be specific for aluminum Be, Zn, and Ga, which behave similarly to aluminum in the fluorescence reaction with morin, do not react with this dyestuff. It is probable that this reaction can be carried out as a spot test. 

A spot test for boric acid is based on this fluorescent reaction. ... 

Zirconium phosphate ion exchange resin (Ionite 3) (H+, Na+ or K+ form) absorbs uraniumvi salts and gives an intense yellow fluorescence under u.v. light. This behavior is used as the basis of a sensitive spot test for uranium. ... 

This paper reports on research involved the design, construction, and evaluation of a portable instrument, a "luminoscope", for detecting skin contamination by coal tars via induced fluorescence. The instrument has been used in the laboratory to measure the fluorescence of various coal tars and recycle solvents from liquefaction processes spotted on filter paper on rat and on hamster skin. The practical use of the devices in field test measurements to monitor skin contamination of workers at coal gasifier is discussed. The paper also discusses the practicality and usefulness of the luminescence method for detecting skin contamination. 

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