Fluorescence spectrophotometry cuvettes

The eluates from sample and standard spots can be analyzed by any convenient and appropriate procedure, for example, titration, electroanalysis, gas chromatography, or spectrophotometry. Ultraviolet, visible, or fluorescence spectrophotometry is most often the method of choice however, densitometry can he carried out directly on the plate in these modes, and the compound will usually be more concentrated on the chromatogram than it will be in solution in a cuvette. In addition, the scraping and elution approach is time-consuming and labor-intensive and subject to losses of material—for example, by irreversible adsorption. The extraction method has been shown to have a reproducibihty that is markedly poorer than with the use of a modem scanning densitometer (Matysik et al., 1994). 

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