Fluorescence Polarization, a Tool for High-Throughput Screening

A typical ITC experiment (for experimental setup and instrumentation see Pierce et al., 1999) consists of sequential injections of a ligand with extremely accurately adjusted volume into the receptor solution. Both the ligand and the receptor have to be solved in exactly the same buffer. It is crucial that the heat of dilution is subtracted by titration of the same ligand into buffer only. 

This information is a prerequisite for the development of cAMP analogs capable of permeating the cell membrane while maintaining their ability to bind to the regulatory subunit with high affinity. 

Fluorescence Polarization, a Tool for High-Throughput Screening 

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