Fluorescence-photochrome immunoassay

The DNP-DAS label bound between two large protein molecules by means of noncovalent binding to an anti-TNP antibody on one side and on the other side via covalent linkage to lysozyme using cyanuric chloride as the cross-linker [10]. (Reproduced with permission from Elsevier.) 

The FPIA method can be potentially used either independently or as a complementary method to enzyme-linked immunosorbent assay (ELISA) and fluorescence polarization immunoassays (FPIA) technique. FCIA does not need polarization equipment and is not markedly influenced by light scattering effects. The FCIA technique can be expanded for analysis of enzymes and receptors including adaptation to fibro-optic techniques. 

Suppermolecules Containing Stilbene and Fluorescent Quenching Croups 

NO flux (in an initial time interval) in tissue sample from rabbit trachea epithelia and pordne trachea epithelia was found to be as 7.9 x lO mol/s x g and 3.0 X 10 mol/s X g, respectively. 

After a modification, it would be possible to monitor nitric oxide in animal organs and, in future, in human body. The high ability of the tiny NO molecule to rapidly diffuse and penetrate would make it possible to monitor NO penetration through the skin. 

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Theoretical considerations and existing experimental data indicate that under certain conditions, the rate of photoisomerization strongly depends on the microviscosity around the isomerized molecule and upon the effect of steric hindrance. In a viscous medium, the apparent rate constant of trans-cis photoisomerization ki o is controlled by the reorganization rate of the process in the medium (Equations 4.2 and 4.3). This method was used for the measurement of fluidity of biological membranes and microviscosity of a specific site of a protein. On this theoretical basis, fluorescence-photochrome immunoassay (FPHIA)] were used. 

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