Fluorescence lifetime imaging FLIM

Schneckenburger, H., Stock, K., Lyttek, M., Strauss, W. S. and Sailer, R. (2004a). Fluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells. Photochem. Photobiol. Sci. 3, 127-31. [Pg.421]

Measurements of fluorescence lifetime ofa chromophore can enhance the potential offluorescence microscopy [1,2,5-8]. Fluorescence lifetime is an inherent property of a chromophore, and thus is independent of chromophore concentration, photo-bleaching and, excitation intensity, but highly dependent on pH, ion concentration, and local environment that affects the non-radiative rate of a chromophore. This makes fluorescence lifetime imaging (FLIM) a powerful tool for quantitative imaging of cellular conditions as well as the circumstances around the fluorescent dyes. [Pg.325]

The multigate technique has become one of the standard techniques of fluorescence lifetime imaging (FLIM) in laser scanning microscopes [145, 185, 186, 439, 519]. The practical implementation of the technique is described in [78, 486]. [Pg.15]

A. Riick, F. Dolp, C. Happ, R. Steiner, M. Beil, Fluorescence lifetime imaging (FLIM) using ps-pulsed diode lasers in laser scanning microscopes, Proc. SPIE 4962, 160-167 (2003)... [Pg.379]

Periasami, A., Elangovan, M., Elliot, E., and Brautigan, D. L. (2002) Fluorescence lifetime imaging (FLIM) of green fluorescent fusion proteins in living cells. Methods Mol. Biol. 183, 189-200. [Pg.198]

Figure 16. Intuitive description of fluorescence lifetime imaging (FLIM).

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