Fluorescence imaging, applications

Chang CJ, Javorski J, Nolan EM, Shaeng M, Lippard SJ (2004) A tautomeric zinc sensor for ratiometric fluorescence imaging application to nitric oxide-release of intracellular zinc. Proc Natl Acad Sci USA 101 1129-1134... 

Laser heating, case hardening by, 16 200 Laser heterodyne radiometry, 23 142 Laser-induced breakdown spectroscopy (LIBS), archaeological materials, 5 743 Laser-induced EDC cracking, 25 646 Laser-induced fluorescence, 23 127 Laser-induced fluorescence imaging, application in combinatorial chemistry, 7 404... 

A series of silica nanoparticles doped with cyanine dye DY-635 (Dyomics) were also prepared, characterized, and investigated in flow cytometry and fluorescence imaging applications [77]. Also these dye-nanoparticles demonstrate high, stable, and tunable fluorescence intensity and are useful for multicolor detection. 

D. Krauzewicz, N. Korchev, Y., Scanning surface confocal microscopy for simultaneous topographical and fluorescence imaging Application to single virus-like particle entry into a cell. Proceedings of the National Academy of Sciences 2002, 99, 16018-16023. 

As soon as fight intensity becomes the limiting factor which is clearly the case for five specimen fluorescence imaging applications, images with similar... 

Ai HW, Henderson JN, Remington SJ, Campbell RE (2006) Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein structural characterization and applications in fluorescence imaging. Biochem J 400 531-540... 

Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE (2008) Hue-shifted monomeric variants of Clavularia cyan fluorescent protein identification of the molecular determinants of color and applications in fluorescence imaging. BMC Biol 6 13. doi 10.1186/1741-2007-1186-1113... 

The use of doped and undoped silica aerogels as multifunctional host materials for fluorescent dyes and other luminescent materials for display and imaging applications has been reported.278 Results have been presented on the PL spectra of undoped silica aerogels and aerogels doped with Er3+, rhodamine, and fluorescein.278... 

Noise can be also introduced by biochemical heterogeneity of the specimen. This can be a major cause of uncertainty in biological imaging. The high (three-dimensional) spatial resolution of fluorescence microscopy results in low numbers of fluorophores in the detection volume. In a typical biological sample, the number of fluorophores in the detection volume can be as low as 2-3 fluorophores for a confocal microscope equipped with a high NA objective at a fluorescent dye concentration of 100 nM. This introduces another source of noise for imaging applications, chemical or molecular noise, related to the inherent randomness of diffusion and the interaction of molecules. 

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