Fluorescence fundamental anisotropy

It should first be noted that the measurement of emission anisotropy is difficult, and instrumental artefacts such as large cone angles of the incident and/or observation beams, imperfect or misaligned polarizers, re-absorption of fluorescence, optical rotation, birefringence, etc., might be partly responsible for the difference between the fundamental and limiting anisotropies. 

In this chapter we describe the fundamental theory for steady-state measurements of fluorescence anisotropy and present selected biochemical applications. In the next chapter we will describe the theory and applications of time-resolved anisotropy measurements. 

For motionless molecules isotropically distributed, r does not depend on time and its value, r, called the fundamental emission anisotropy is characteristic of the fluorescent molecule considered ... 

Fundamental Fluorescence Anisotropy in Time-Resolved Measurements... 

The studies presented in this chapter clearly demonstrate the potential that exist for using fluorescence anisotropy to study chiral recognition in CD-based systems, which has ramifications from both fundamental and applied perspectives. For example, preliminary work in our laboratory has already shown the potential of using the system for evaluating and optimizing chiral separation system a priori [39]. Other areas worthy of pursuit lie in further investigation of the method for determination of enantiomeric excess and the determination of enantioselective thermodynamic parameters, primarily in terms of increasing the precision and sensitivity of the measurements. 

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