Fluorescein diphosphate

In a further extension, they demonstrated screening of submicroliter volumes with fluorescein diphosphate (FDP) as substrate against multiple enzymes (lysozyme, RNase, catalase and alkaline phosphatase) using preformed arrays of nanoliter plugs in a three-phase (liquid-liquid-gas) plug flow (Scheme 4.99) [418],... 

Huang ZJ, Olson NA, You WM, et al. 1992. A sensitive competitive elisa for 2,4-dinitrophenol using 3,6-fluorescein diphosphate as a fluorogenic substrate. J Immunol Methods 149 261-266. 

Phosphatase substrates (classic chro-mogenic sustrates BCIP and NBT. Novel fluorescent substrates Fluorescein diphosphate, Dimethyl-acridi-none phosphate, methylumbelliferyl Phosphates, ELF 97 e.o.) biolumines-cent determination of ATP ... 

Rapid and facile generation of capsules from tandem assembly in aqueous media is amenable to encapsulation of water-soluble compounds. Encapsulation of ICG dye within PAH/H2PO4 aggregates was shown by Yu et al. Enzyme encapsulation and the feasibility of capsules to serve as reaction vessels was demonstrated by Rana et al. In their study, they encapsulated acid phosphatase enzyme in PLL-citrate-silica sols and suspended the spheres in a solution containing fluorescein diphosphate. Fluorescence increased in intensity within the shell walls as fluorescein was formed by enzymatic cleavage of phosphate groups. This study showed that microcapsules could serve as reaction vessels that allow enzymatic action to take place in a protective environment and allow for reactants and/or products to diffuse through permeable shell walls. 

Phospholipase D from Streptomyces sp. AA586 was obtained from Genzyme Diagnostics (West Mailing, UK), Naja naja phospholipase A2 was purchased fix>m Sigma (USA), and fluorescein diphosphate from Molecular Probes (USA). 

In a series of experiments it has been shown that it is possible to prepare giant POPC vesicles by the electroformation method in the presence of (non-fluorescent) fluorescein diphosphate, a substrate for alkaline phosphatase. The vesicles thus formed contained in the interior aqueous space substrate molecules which, after... 

Abbreviations EPR, electron paramagnetic resonance FITC, fluorescein-5 -isothiocyanate lAEDANS, iV-iodoacetyl-N -(5-sulfo-l-naphthyl)ethylenediamine NCD, fluorescent yV-cyclohexyl-N -(4-dimethyl-amino-a-naphthyl)carbodiimide RITC, rhodamine-5 -isothiocyanate DPPE, dipalmitoylphosphatidyl-ethanolamine PE, egg phosphatidyl-ethanolamine ANS, 8-anilino-l-naphthalene sulfonate DPH, diphenylhexatriene e-ADP, l,iV -ethanoadenosine-5 -diphosphate TNP-ADP, 2 [3 ]-0-(2,4,6-trinitrophe-nyl)adenosine-5 -diphosphate. 

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