Fluorescein dilaurate test

The pancreolauryl test (fluorescein dilaurate test, Temmler Pharma, Germany) is similar in its principle to the NBT-PABA test. Fluorescein dilaurate (FDL) is poorly water-soluble. It is given orally in the middle of a standard breakfast (50 g white bread, 20 g butter, and one cup of tea) and is specifically hydrolyzed by pancreatic cholesterol esterase, which requires bile salts for its activity. The released... 

Figure 9 The pancreolauryl test allows an indirect assessing of exocrine pancreatic function. Orally administered fluorescein dilaurate is hydrolyzed by carboxylester lipase (identical to cholesterol esterase) liberating lauric acid and free, water-soluble fluorescein. The latter is readily absorbed in the small intestine, partly conjugated in the liver, and excreted in urine mainly as fluorescein glucuronide. By measuring the concentration of fluorescein in the urine over a period of, for instance, 10 hours, the total quantity of this dye is determined. (From Ref. 52.)...

A periodic measurement of stool fat excretion by means of the method of Van de Kamec [97] or using the less unpleasant near-infrared analysis [98-100] allows quantitative evaluation of the degree of steatorrhea. Besides this, several function tests where the pancreas is directly stimulated by an infusion of secretin and/or cholecystokinin (reviewed in Ref. 101) or indirect tests requiring the administration of an exogenous pancreas enzyme substrate as V-benzoyl-L-tyrosyl-p-aminobenzoic acid (NBT-PABA) or fluorescein dilaurate (pancreo-lauryl test) (Fig. 12) are available. The NBT-PABA test measures intraluminal... 

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