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Fluorescamine assay for the availability of side-chain amino groups [Pg.225]

Proteins adjacent to peroxidizing phospholipids may undergo modification by the interaction of the free amino groups of the side-chains of the substituent lysine residues with a range of aldehydic metabolites of lipid hydroperoxide breakdown. [Pg.225]

Alternatively, free amino groups of lysine side-chains may be oxidized. Thus the fluorescamine assay is used to measure the extent of loss of amino groups in proteins. [Pg.225]