9-Fluorenylmethyl chloroformate chloride

A derivatization with acid chlorides is also possible. Amino acids can be derivatized with 9-fluorenylmethyl chloroformate (FMOC) and separated on a CSP with X-cyclodextrin (ChiraDex gamma ), a cyclic oligosaccharide which consists of eight glucose units. 

A comparative study was made of the RP-HPLC analysis of free amino acids in physiological concentrations in biological fluids, with pre-column derivatization by one of the four major reagents o-phthalaldehyde (73) in the presence of 2-mercaptoethanol, 9-fluorenylmethyl chloroformate (90), dansyl chloride (92) and phenyl isothiocyanate (97, R = Ph) (these reagents are discussed separately below). Duration of the analysis was 13-40 min. Sensitivity with the latter reagent was inferior to the other three however, its use is convenient in clinical analysis, where sample availability is rarely a problem. The derivatives of 73 were unstable and required automatized derivatization lines. Only 92 allowed reliable quantation of cystine. All four HPLC methods compared favorably with the conventional ion-exchange amino acid analysis188. 

Since BAs occurring in food do not exhibit satisfactory absorbance or fluorescence in the visible or ultraviolet range, chemical derivatization, either pre- (35-37) or postcolumn (38), is usually used for their detection in HPLC. The most frequently employed reagents for precolumn derivatization are fluorescamine, aminoquinolyl-lV-hydroxysuccinimidyl carbamate (AQC) (39, 40), 9-fluorenylmethyl chloroformate (FMOC) (41-43), 4-dimethylaminoazobenzene-4 -sul-fonyl chloride (dabsylchloride, DBS) (44), N-acetylcysteine (NAC) (45,46), and 5-dimethyl-amino-1-naphthalene-1-sulfonyl chloride (dansylchloride, DNS) (47,48), phthalaldehyde (PA), and orf/to-phthaldialdehyde (OPA) (49-51), together with thiols such as 3-mercaptopropionic acid (MPA) (37) and 2-mercaptoethanol (ME) (35,49). 

Both primary and secondary ammo acids react with 9-fluorenylmethyl chloroformate (FMOC chlonde) under alkaline, aqueous conditions (Anson-Moye and Boning, 1979). The reaction is complete in 30 s. However, although FMOC chloride is fluorescent, the excess reagent and the byproducts are easily removed by extraction prior to separation (Ejnarsson et al., 1983). 

Related Reagents. 9-Fluorenylmethyl Chloroformate 2-Methylsulfonyl-3-phenyl- l-prop-2-enyloxycarbonyl Chloride. ... 

Related Reagents. Allyl Chloroformate 4-Bromobenzyl Chloroformate f-Butyl Chloroformate Ethyl Chloroformate 9-Fluorenylmethyl Chloroformate Isobutyl Chloroformate Methyl Chloroformate 2,2,2-Tribromoethyl Chloroformate 2,2,2-Trichloroethyl Chloroformate 2,2,2-Trichloro-r-butoxycarbonyl Chloride 2-(Trimethylsilyl)ethyl Chloroformate Vinyl Chloroformate. 

Related Reagents. Allyl Chloroformate Benzyl Chlo-roformate 4-Bromobenzyl Chloroformate t-Butyl Azidoformate l-(t-Butoxycarbonyl)-lEf-benzotriazole 3-A-oxide 1 -(r-Butoxycarbonyl)imidazole 2-(t-Butoxycarbonyloxyimino)-2-phenylacetonitrile A-(t-Butoxycarbonyloxy)phthalimide A-(t-Butoxycarbonyloxy)succinimide V-(r-Butoxycarbonyl)-1,2,4-triazole Di-t-butyl Dicarbonate Ethyl Chloroformate 9-Fluorenylmethyl Chloroformate Isobutyl Chloroformate Methyl Chloroformate 2,2,2-Tribromoethyl Chloroformate 2,2,2-Trichloroethyl Chloroformate 2,2,2-Trichloro-t-butoxycarbonyl Chloride 2-(Trimethylsilyl)ethyl Chloroformate Vinyl Chloroformate. 

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