Flow injection mass spectrometry

Wang, T. Zeng, L. Strader, T. Burton, L. Kassel, D. B. 1998. A new ultra-high throughput method for characterizing combinatorial libraries incorporating a multiple probe autosampler coupled with flow injection mass spectrometry analysis. Rapid Commun. Mass Spectrom., 12, 1123-1129. 

LC/MS emerged as the method of choice for the quality control assessment to support parallel synthesis because the technique, unlike flow injection mass spectrometry, provides the added measure of purity (and quantity) of the compound under investigation. In addition, universal-like HPLC gradients (e.g., 10% to 90% acetonitrile in water in 5 minutes) have been found to satisfy the separation requirements for the vast majority of combinatorial and parallel synthesis libraries. Fast HPLC/MS has been found to serve as good surrogate to conventional HPLC for assessing library quantity and purity [34-37]. Fast HPLC/MS is simple in concept. It involves the use of short columns (typically 4.6 mm i.d. x 30 mm in length) operated at elevated flow rates (typically 3-5mL/min). 

Recent reports by Kyranos et al. [38] suggest that pseudo-chromatography (in essence, step elution chromatography) provides a more rapid and reliable assessment of the quality of library synthesis than methods such as flow injection mass spectrometry. 

Morand, K.L. Burt, T.M. Regg, B.T. Chester, T.L. Techniques for Increasing the Throughput of Flow Injection Mass Spectrometry, Anal. Chem. 73,247-252 (2001). 

Lane SJ, Tucker MG (1998) An automated electroosmotic sample introduction/separation interface for automated electroosmotic flow flow-injection mass spectrometry, infusion MS and CEC MS. Rapid Commun Mass Spectrom 12 947-954... 

Nanita, S. C. 2011. High-throughput chemical residue analysis by fast extraction and dilution flow injection mass spectrometry. Analyst 136 285-287. 

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