Floating section immunocytochemistry

Floating section immunocytochemistry - performed on free-floating, thick section (25-100 mm) cut on a Vibratome or freezing microtome with antibodies penetrating deeper because of the greater movement of the section floating both sides of the section will be exposed to the antibodies. 

Shiurba RA, Spooner ET, Ishiguro K, et al. Immunocytochemistry of formalin-fixed human brain tissues microwave irradiation of free-floating sections. Brain Res. Brain Res. Protoc. 1998 2 109-119. 

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