FLIPR imaging

Fig. 3. Comparison between IP-One kit versus calcium mobilization assay (384-well format). Human embryonic kidney (HEK) 293 cells expressing a chemokine receptor were evaluated on HTRF IP-One kit (CisBio, Bedford, MA) and fluroescent imaging plate reader (FLIPR) with Calcium 3 kit (Molecular Devices, Mountain View, CA).

Fluorescent, voltage-sensitive dyes can be employed to investigate the activity of ion channels by reflecting the cellular membrane potential (Epps et al. 1994 Plasek and Sigler 1996). This approach is suitable for use in conjunction with different measurement systems including Fluorometric imaging plate readers (FLIPR), Voltage/Ion probe readers (VIPR) or conventional fluorescence readers. 

Wood M, Smart D. Real time receptor function in vitro micro-physiometry and the fluorometric imaging plate reader (FLIPR). In Receptors Structure and Function. 2nd edition. Stanford C, Horton R, eds. 2001. Oxford University Press, Oxford, pp. 175-191. 

The impact of different pharmacological compounds has been evaluated on hESC- or iPS-derived cardiomyocytes using Image Xpress Micro and FLIPR Tetra systems [111], impedance-based xCELLigence real-time cell analyzer Cardio system [112, 113], MEA [54, 114], and perforated patch clamp [38], The impact of cardiotoxic drugs on the release of biomarkers, such as troponin T and fatty acid-binding protein 3 from cardiomyocytes, has also been investigated to predict the cardiotoxicity [115]. 

The AequoScreen system developed by Euroscreen utilizes this biolumines-cent Ca -indicator in cell-based assays. This system offers an interesting alternative to the traditional, fluorescence-based assays for intracellular Ca measurements (see Section 10.3.1 10.3.1 Fluorimetric Imaging Plate Reader (FLIPR) ). 

The fluorimetric imaging plate reader (FLIPR , Molecular Devices) system is a highly specialized, advanced plate reader that is able to produce a kinetic readout for fluorimetric, cell-based assays in a homogeneous format. Examples of such assays are measurements of intracellular concentration, membrane potential,... 

Abbreviations FLIPR, fluorescence imaging plate reader VIPR, voltage ion probe reader. 

Behrendt HJ, Cermann T, Cillen C, Hatt H, Jostock R (2004) Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VRl using a fluorometric imaging plate reader (FLIPR) assay. Br J Pharmacol l4l ... 

Fluorometric Imaging Plate Reader (FLIPR) was from Molecular Devices (Sunnyvale, CA). Common tissue culture reagents were from Gibco/BRL (Rockville, MD). The 384-well plates used were from Costar (Cambridge, MA cat. no. 3712). 

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