Fluorometric Imaging Plate Reader

Fluorescent, voltage-sensitive dyes can be employed to investigate the activity of ion channels by reflecting the cellular membrane potential (Epps et al. 1994 Plasek and Sigler 1996). This approach is suitable for use in conjunction with different measurement systems including Fluorometric imaging plate readers (FLIPR), Voltage/Ion probe readers (VIPR) or conventional fluorescence readers. 

Wood M, Smart D. Real time receptor function in vitro micro-physiometry and the fluorometric imaging plate reader (FLIPR). In Receptors Structure and Function. 2nd edition. Stanford C, Horton R, eds. 2001. Oxford University Press, Oxford, pp. 175-191. 

Behrendt HJ, Cermann T, Cillen C, Hatt H, Jostock R (2004) Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VRl using a fluorometric imaging plate reader (FLIPR) assay. Br J Pharmacol l4l ... 

Fluorometric Imaging Plate Reader (FLIPR) was from Molecular Devices (Sunnyvale, CA). Common tissue culture reagents were from Gibco/BRL (Rockville, MD). The 384-well plates used were from Costar (Cambridge, MA cat. no. 3712). 

Sullivan, E. Ihcker, E. M. Dale, 1. L. Measurement of [Ca ] using the fluorometric imaging plate reader (FLIPR). Methods Mol. Biol. 1999,114, 125-133. 

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