Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

FLIPR assay

The truth most likely lies somewhere in between. Bender [67] published the most quantitative study to date on the success of HTS at Novartis. Several conclusions could be drawn. Particular target types and assay technologies have a great impact on screening success, and this was not always correlated to the number of identifying hits in the HTS runs. For assay formats used a minimum of five times, LC/MS readouts succeed 83% of the time, followed by FP assays, which succeed in 72% of the cases. TR-FRET showed a success rate of 70%, with FLIPR assays (61%), fluorescence intensity readouts (59%), and AlphaScreen (60%) performing... [Pg.59]

A series of 4,4-disubstituted quinazolin-2-ones derived from HTV nonnucleoside reverse transcriptase inhibitor leads have shown good in vitro potency and in vivo efficacy [28]. Using FLIPR assays on cell lines with different resting membrane potentials, TTA-Q3 (10) and TTA-Q6 (11)... [Pg.9]

Question A methyl analogue (R = Me) of a muscarinic competitive antagonist shows a pA2 value of 9.55 in the FLIPR assay with a 20 min preincubation of cells with antagonist. All SAR in this series indicate that increasing... [Pg.337]

Question This question is based on data from the previous question. A perspicacious chemist recalled that the R = Me compound produced noncompetitive antagonism in the FLIPR assay. The pattern of the curves (control and curve in the presence of 5 nM antagonist after 2 hours equilibration) is shown in Figure 14.14A. This produced consternation among the chemist ranks until the biologist assured them it was expected. The chemists then asked what the pattern was for the R = tBut compound. If the... [Pg.340]

Behrendt HJ, Cermann T, Cillen C, Hatt H, Jostock R (2004) Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VRl using a fluorometric imaging plate reader (FLIPR) assay. Br J Pharmacol l4l ... [Pg.31]

Fiuo-4 Method for FLIPR Assay to Detect Caicium Mobilization Proprietary GPCR... [Pg.245]

See other pages where FLIPR assay is mentioned: [Pg.6]    [Pg.10]    [Pg.13]    [Pg.14]    [Pg.442]    [Pg.140]    [Pg.368]    [Pg.336]    [Pg.337]    [Pg.338]    [Pg.339]    [Pg.339]    [Pg.340]    [Pg.313]    [Pg.330]    [Pg.232]    [Pg.108]   
See also in sourсe #XX -- [ Pg.6 , Pg.9 , Pg.10 , Pg.13 , Pg.14 , Pg.442 ]

See also in sourсe #XX -- [ Pg.228 ]



SEARCH



FLIPR

© 2024 chempedia.info