Flavopiridol glucuronide

Villeneuve L, Girard H, Fortier LC et al. Novel functional polymorphisms in the UGT1A7 and UGT1A9 glucuronidating enzymes in Caucasian and African-American subjects and their impact on the metabolism of 7-ethyl-10-hydroxycamptothecin and flavopiridol anticancer drugs. J Pharmacol Exp Ther 2003 307 117-128. 

Jager, W., Gehring, E., Hagenauer, B.,Aust, S., Senderowicz, A., and Thalhammer, T. (2003) Bihary excretion of flavopiridol and its glucuronides in fhe isolated pei fiised rat liven role of multidrug resistance protein 2 (Mrp2). Life Sciences, 17, 2841-2854. 

The metabolism of flavopiridol by the liver has been studied both in vitro and in vivo. Glucuronidation was found to occur when isolated human liver microsomes were used [33] and this was confirmed by examination of the results from a perfused rat liver [34] which showed that the process occurred at both the 5 and 7 position and that the metabolites were excreted mainly into the bile. A reverse-phase HPLC analytical method has been devised for analysis of flavopiridol in serum [35]. After extraction with dichloromethane and subsequent evaporation, the compound and internal standard are dissolved in the mobile phase and separated using a C-l 8 column with a mobile phase of methanol water 1 1 containing 0.05M ion-pairing reagent PIC B-6. The compounds were detected at 278nm. 

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