Fixation procedures tissues

The extent to which these new tissue adhesives and hemostats will influence surgical procedures can be seen in the following analogy. An effective carpenter cannot create a masterpiece without a saw to cut wood, nails to ensure local fixation of separate pieces of wood, and glue to cause broad surface apposition between wood surfaces. Similarly, the surgeon presently uses a scalpel to cut and divide tissues as well as a suture to create local fixation of tissues, but until... 

As mentioned, chemical fixation of plant cells has been reviewed many times (15-20) and the reader is referred to these citations for a variety of fixation procedures for preserving plant cells and tissues. One of the most recent references regarding the topic is that of Hopwood and Milne (21). Table 1 presents their recommendations regarding fixation of plant cells and tissues for electron microscopy. 

Practically speaking, that would mean that overnight fixation would be required, that weekend fixation would be OK, but a longer fixation time would not be useful. With this information, users would be able to evaluate the results obtained from tissues received from outside sources by comparing fixation procedures. 

Fixation is the stabilization or preservation of cells and tissue as close to life-like as possible. All fixation procedures change the tissue they are preserving, but the key is to find the least amount of change for immunocytochemistry. 

To evaluate the success of a particular fixation procedure, the hybridized sample should be compared to identically fixed tissue that has not undergone FISH. In particular, the appearance of condensed mitotic or meiotic chromosomes with and without hybridization can provide a usefiol criterion for deciding whether preservation is adequate (Figure 2.3). Underfixation will result in deterioration of morphology overfixation will... 

Microwaves have successfully been used for rewarming of blood for medical appHcations (157). Another successful appHcation, not commetciali2ed as of this writing, is the use of microwave heating for rapid tissue fixation (158,159). This procedure appears to reduce the time for tissue sample analysis... 

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