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Firefly luciferase substrate specificity

The firefly luciferase (EC 1.13.12.7) catalyzes the emission of light in the presence of ATP, Mg, molecular oxygen and firefly luciferin a specific natural substrate (Figure 3). [Pg.161]

Figure 3. (a) The firefly luciferase bioluminescence reaction, (b) Structure of the specific substrate luciferin and the corresponding reaction product oxyluciferin. [Pg.161]

Two types of bioluminescence can be characterized firefly-QL, and bacterial-BL,. BL of living systems is extremely bright because each molecule of the product is formed in an electronically-excited state. The quantum yield of BL reactions is extremely high (0.1-1.0) compared to the CL of non-enzymatie oxidized organic matter. Hence, high efficiency is reached by the application of highly specific enzymes - luciferases. These are especially effective for their substrates — luciferins, which is one of the main conditions needed for chemical redox energy transformations into... [Pg.230]

See other pages where Firefly luciferase substrate specificity is mentioned: [Pg.205]    [Pg.640]    [Pg.65]    [Pg.240]    [Pg.257]    [Pg.53]    [Pg.55]    [Pg.519]    [Pg.5]    [Pg.6]    [Pg.135]    [Pg.1228]    [Pg.579]    [Pg.218]    [Pg.573]    [Pg.280]    [Pg.1224]    [Pg.135]    [Pg.98]   
See also in sourсe #XX -- [ Pg.641 ]



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Substrate specificity

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