Final method stepwise procedure

A written stepwise analytical procedure is a document that provides a detailed description of the extraction and analysis techniques used for validation (Chapter 10). If the method has been previously validated, the stepwise procedure will be a component of the final method or SOP. Many laboratories will in fact create a draft method... 

The description of the assay, including a detailed stepwise procedure that was established during method development should be followed exactly as written during the validation and ultimately documented in a final analytical method or SOP. A description of the specific documentation requirements for the method can be found in Section 9.8.5. The method may also include references to other laboratory SOPs that describe routine procedures (e.g. equipment calibration). 

The present article will be devoted in its first chapters to the chemical basis of the stepwise degradation procedure and to a comparative description of design and function of the two instruments, and in its later chapters to the results so far achieved. Finally, present limitations of the automated method as well as novel approaches to the sequence analysis of peptides will be discussed. 

The peptide synthesis consists of stepwise-condensation and segment-condensation procedures. In both procedures, it is possible to purify the reaction product at each reaction step. Therefore, protected peptides with a high degree of purity can be obtained from a solution method. The choice of the reagents used in the final deprotection step determines the main strategy of peptide synthesis. The final deprotection procedures are mainly (1) catalytic hydrogenolysis in the presence of palladium,(2) sodium treatment in liquid ammonia for 10 to 15 seconds, repetitively over 30 minutes,(3) TFA treatment at room temperature for 1 hour,t l (4) HF treatment at 0°C for 1 hour,t l or (5) hard-soft acid-base procedure.0 ... 

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