Filtration setup

It will be a little tricky but one can also try to purify by freezing I The sassafras oil is thrown into the freezer to chill. Safrole itself freezes at -14°C so anything that starts to freeze prior to that can be cold filtered in a prechilled vacuum filtration setup. The filtrate goes back in the freezer until -14°C is reached and the mother lode of safrole freezes up. This again is filtered cold but this time the frozen mass of safrole crystals are washed with some ice cold methanol or ethanol (preferably at -14°C) to wash away the unfrozen high-boiling constituents. 

After 3 hours the stirring is stopped and the solution allowed to settle. By this time just about all the foil will have turned to dust, which is going to make the next step of vacuum filtration very difficult because it will plug up the filter paper in a second. So the chemist lets it settle, then pours off the liquid through the vacuum filtration setup (see methodology section). The flask is rinsed with lOOmL methanol, the methanol poured through the grey filter cake and the filter cake discarded. All of the filtrate is placed in a flask and vacuum distilled to remove all the methanol, isopropyl alcohol and water which will leave the chemist with oil and junk in the bottom of the flask. 

Fig. 15. Production ready Quadra96 with scavenger resin filtration setup. (See color insert.)...

Figure 6.2 Vacuum filtration setup using the Buchner funnel.

FIGURE 8.29 Schematics of the filtration setup with collapse-tube pulsation generator. (Adapted from Figure 1 of Bertram, C.D., Hoogland, M.R., Li, H., Odell, R.A., and Fane, A.G., J. Membr. Sci., 84, 279, 1993.)... 

Fig. 59 The gravity filtration setup with a funnel that fits the iron ring.

Dechorionate embryos in 50% bleach (Clorox) using a Millipore filtration setup as described in step 1 of Protocol 14.4. Resuspend dechorionated embryos in a small volume of PBS-Triton and transfer to a 20-ml screw-cap vial. 

---