Fibronectin isolation

Sekiguchi, K., Siri, A., Zardi, L., and Hako-mori, S. (1985) Differences in domain structure between human fibronectins isolated from plasma and from culture supernatants of normal and transformed fibroblasts. Studies with domain-specific antibodies, J Biol Ghem 260, 5105-5114. 

Froman, G., Switalski, L., Speziale, P., and Hook, M. (1987). Isolation and characterization of a fibronectin receptor from Staphylococcus aureus, f. Biol. Chem. 262,6564-6571. 

Jnng M, Lee HC, Ahn CW, Park W, Choi W, Kim H, Cho D, Lee GT, Li H-R. (2002) Effective isolation of magnesinm lithospermate B and its inhibition of aldose rednctase and fibronectin on mesangial cell line. Chem Pharm Bull 50 1135-1136. 

Recently, TTR1 fibrils have been decorated with the classic RGD tripeptide motif isolated from fibronectin, which encourages cell adhesion via integrin cell surface receptors (Gras et al., 2008). This bioactive tag was added to the TTR1 sequence which drives fibril assembly and the tag shown to be exposed on the fibril surface and accessible to cells following assembly. The RGD-modified fibrils were bioactive in a cell dissociation assay which measures the ability of fibrils to competitively bind to cells and induce cell detachment from a surface, as illustrated in Figure 19. In... 

Fig. 10 Viability of human endothelial cells isolated from umbilical cord on unmodified, RGD-modified gels (here shown for Type B) and fibronectin (FN) reference monitored by the colorimetric MTT-(tetrazolium)-assay. MTT-assay was used to quantify living cells after 3 days in culture...

Erickson, H.P. and Inglesias, J.L. (1984) A six-armed oligomer isolated from cell surface fibronectin preparations. Nature (London) 311 267-269. 

Protein Purification. Canine plasma fibronectin was used in this study in order to correlate these in vitro studies with canine ex vivo experiments involving preadsorbed canine proteins. Canine FN was isolated from citrated canine plasma using the methods of Ruoslahti (11). The FN was suspended in phosphate buffered saline (PBS) containing 0.02% NaN3, and then snapfrozen and stored at -70 C until less than 24 hours before use. The protein was then snapthawed at 40 C, filtered (0.22 ym Millex GV, Millipore,... 

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