Fibre optics immunosensors

Electrochemical immunosensors are the most numerous of immunosensors. Their suitability for use in flow systems is limited by the two factors commented on above in dealing with fibre optic-based sensors, viz. the need for an incubation period and exceedingly long response times. 

Some electrochemically active substances that can generate photons on an electrode surface are suitable labels for homogeneous immunoassays. A labelled antigen exhibits an electrochemical reactivity and produces luminescence, but when it is immunochemically complexed, the labelled antigen loses its electrochemiluminescent properties. One optical immunosensor for homogeneous immunoassays was assembled by spattering platinum on the end surface of an optical fibre. Spattered platinum maintains optical transparency and functions as an electrode. An optical electrode efficiently... 

Lemmer K, Donoso Mantke O, Bae HG, Groen J, Drosten C, Niedrig M (2004) External quality control assessment in PCR diagnostics of dengue virus infections. J Clin Virol 30(4) 291-296 Leshem B, Sarfati G, Novoa A, Breslav I, Marks RS (2004) Photochemical attachment of biomolecules onto fibre-optics for construction of a chemiluminescent immunosensor. Luminescence 19(2) 69-77... 

Fig. 4 The future immunosensor with fibre optic (a) and reaction with mycotoxin (b)...

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