Fersht, Alan

Library of Congress Cataloging in Publication Data Fersht, Alan, 1943-... 

Vaughan, Cara K., Harryson, Pia, Buckle, Ashley M., Fersht, Alan R. (2002), Acta Crystallographica, Section D Biological Crystallography D58, 591-600... 

Recently Alan Fersht, Cambridge University, has developed a protein engineering procedure for such studies. The technique is based on investigation of the effects on the energetics of folding of single-site mutations in a protein of known structure. For example, if minimal mutations such as Ala to Gly in the solvent-exposed face of an a helix, destabilize both an intermediate state and the native state, as well as the transition state between them, it is likely that the helix is already fully formed in the intermediate state. If on the other hand the mutations destabilize the native state but do not affect the energy of the intermediate or transition states at all, it is likely that the helix is not formed until after the transition state. 

Structure and mechanism in protein science a guide to enzyme catalysis and protein folding / Alan Fersht. p. cm. 

Source From Alan Fersht, Enzyme Structure and Mechanism, 3d ed. Copyright 1985 by W. H. Freeman Company, New York. Reprinted with permission. 

Alan Fersht, Structure and Mechanism in Protein Science, Freeman, New York, 1999. 

Alan Fersht, Structure and Mechanism in Protein Science, Freeman, New York, 1999. Roberta. Copeland, Enzymes - A Practical Introduction to Structure, Mechanism, and Data Analysis, Wiley-VCH, New York, 1996. 

It is also possible now to write protein sequences the approach, called protein engineering, was pioneered in 1980s by Sir Alan Fersht in the University of Cambridge in Great Britain. His idea was to use a natural system of biosynthesis that is at work in every living cell in this sense, it is the entire cell that is now a technological device. Given our ability to prepare any DNA sequence, in principle, we could get a cell to produce protein molecules with any primary structure we wish. However, even if the technical side were no problem, the trouble is that no one really knows what to wish for. When the total number of units N 10, and there are 20 candidates for... 

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