Femtosecond fluorescence dynamics imaging

D. Application 2 Femtosecond Fluorescence Dynamics Imaging EROM Tetracene-Doped Anthracene Microcrystal... 

The second example of the application of fluorescence up-conversion microscope is imaging of organic microcrystals based on ultrafast fluorescence dynamics (femtosecond fluorescence dynamics imaging) (Fujino et al. 2005a). In this measurement, the site-specific energy transfer rate in a tetracene-doped anthracene microcrystal was measured, and the crystal was visualized based on the observed local ultrafast dynamics. 

FIGURE 3.7 (a) The CCD camera image of the tetracene-doped anthracene microcrystal used for the femtosecond fluorescence dynamics imaging, (b) The dynamics image obtained from the region indicated by a broken rectangle in (a) (excitation 400 nm fluorescence 530 nm). (From Fujino, T., Fujima, T., and Tahara, T., J. Phys. Chem. B 109 15327-15331, 2005. Used with permission.)... 

Fujino, T, Fujima, T, and Tahara, T. 2005. Femtosecond fluorescence dynamics imaging using a fluorescence up-conversion microscope. J. Phys. Chem. B 109 15327. 

As described in the previous section, the femtosecond fluorescence up-conversion microscope enabled us to visualize microscopic samples based on position-depen-dent ultrafast fluorescence dynamics. However, in the imaging measurements using the fluorescence up-conversion microscope, XY scanning was necessary as when using FLIM systems. To achieve non-scanning measurements of time-resolved fluorescence images, we developed another time-resolved fluorescence microscope. 

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