Fatty acid w-hydroxylation

Fatty acid w-hydroxylation is involved in the.metabolism of prostaglandins and leukotrienes, and is the first step of dicarboxylic acid formation in the cell. In this assay, lauric acid is hydroxylated to form 12-hydroxylauric acid, which is then fluorescence-labeled on the carboxyl group with 3-bromomethyl-7-methoxy-l,4-benzoxazin-2-one. 

CYP4A2 catalyzes fatty acid w-hydroxylation, but does not catalyze testosterone hydroxylation. 15p-hydroxyIation of steroid sulfates. CYP2C12 also catalyzes weak testosterone I5a- and la-hydroxylase activities. 

K. Nettesheim, E. Wisman etal. (2001). Functional analysis of the LACERATA gene of Arabidopsis provides evidence for different roles of fatty acid w-hydroxylation in development. Proc. Natl. Acad. Sci. USA 98, 9694-9699. 

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