Intestine, fatty acid-binding proteins

Sacchettini, J.C., et al. Refined apoprotein stmcture of rat intestinal fatty acid binding protein produced in Escherichia coli. Proc. Natl. Acad. Sci. USA 86 7736-7740, 1989. 

Actual and predicted structures of three domains of intestinal fatty acid binding protein... 

The hypothesis of the participation of those cholesterol transporters (NPCILI and ABCAl) in the carotenoid transport remains to be confirmed, especially at the in vivo human scale. If the mechanism by which carotenoids are transported through the intestinal epithelial membrane seems better understood, the mechanism of intracellular carotenoid transport is yet to be elucidated. The fatty acid binding protein (FABP) responsible for the intracellular transport of fatty acids was proposed earlier as a potential transporter for carotenoids. FABP would transport carotenoids from the epithelial cell membrane to the intracellular organelles such as the Golgi apparatus where CMs are formed and assembled, but no data have illustrated this hypothesis yet. 

A. Properties of the Intestinal Fatty Acid Binding Protein. 103... 

Corsico, B., Cistola, D.P., Frieden, C. and Storch, J. (1998) The helical domain of intestinal fatty acid binding protein is critical for collisional transfer of fatty acids to phospholipid membranes. Proceedings of the National Academy of Sciences USA 95,12174-12178. 

Hsu, K.-T. and Storch, J. (1996) Fatty acid transfer from liver and intestinal fatty acid-binding proteins to membranes occurs by different mechanisms. Journal of Biological Chemistry 271,13317—13323. 

Sacchettini, J.C. and Gordon, J.I. (1993) Rat intestinal fatty acid binding protein a model system for analyzing the forces that can bind fatty acids to proteins. Journal of Biological Chemistry 268, 18399—18402. 

Figure 13.2 Schematic representation of the creation of hPXR humanized mice. The humanization was achieved in the liver only when the liver-specific albumin promoter was used to direct the transgene expression, or in both the liver and the intestine when the fatty acid binding protein promoter was used. PCN, pregnenolone-16a-carbonitrile RIF, rifampicin. + and mean induction and lack of induction, respectively.

CaM), of fatty acid carboxylate to intestinal fatty acid binding protein (IFABP), and of peptides (e.g, melittin) to Ca +-saturated calmodulin (holo CaM)]. We also extended PLIMSTEX to protein-protein interactions involving self associations of various insulins [33]. These are widely studied systems, and their individual K values range from to 10 M h... 

Intestinal fatty acid-binding protein (lEABP) belongs to a family of 15 IcDa, predominately y -sheet proteins that bind a diverse group of polar lipids [49]. lEABP... 

Cistola, D.P. Kim, K. Rogl, H. Erieden, C. Eatty acid interactions with a helix-less variant of intestinal fatty acid-binding protein. 

Kurian, E. Kirk, W.R. Prendergast, E.G. Affinity of fatty acid for rRat intestinal fatty acid binding protein further examination. Biochemistry 1996, 35, 3865-3874. 

Ogbay, B. Determinants of Stability and Stoichiometry in Intestinal Fatty Acid Binding Protein, PhD Dissertation, Washington University, St. Louis, 2003, 197 pp. 

Katongole, J.B.D. and March, B.E. (1980) Fat utilization in relation to intestinal fatty acid binding protein and bile salts in chicks of different ages and different genetic sources. Poultry Science 59,819-827. 

Hohmann AG, Suplita RL, Bolton NM, Neely MH, Fegley D, Mangieri R, Krey JF, Walker JM, Holmes PV, Crystal JD, Duranti A, Tontini A, Mor M, Tarzia G, Piomelli D (2005) An endocannabinoid mechanism for stress-induced analgesia. Nature 435(7045) 1108-12 Howlett AC, Qualy JM, Khachatrian LL (1986) Involvement of Gi in the inhibition of adenylate cyclase by cannabimimetic drugs. Mol Pharmacol 29(3) 307-13 Hsu K-T, Storch J (1996) Fatty acid transfer from liver and intestinal fatty acid-binding proteins to membranes occurs by different mechanisms. J Biol Chem 271(23) 13317—23... 

In the intestine fatty acid translocase (FAT), fatty acid transporting protein (FATP4) and fatty acid binding protein (FABPpm) have been identified (for review, see Stahl 2003). Natural substrates are long chain fatty acids like myristate, oleate, palmitoate. 

Intestinal fatty acid-binding protein IFABP 1.2 Scapin et al. (1992)... 

Intestinal fatty acid-binding protein 2 IFABP2 Not determined Kandario/. (1991)... 

Li, H. and Frieden, C. (2005) NMR studies of 4-F-19-phenylalanine-labeled intestinal fatty acid binding protein Evidence for conformational heterogeneity in the native state. Biochemistry, 44(7), 2369-2377. 

Hodsdon, M. E. and Cistola, D. P. (1997) Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by N-15 NMR relaxation and H-l exchange. Biochemistry, 36(8), 2278-2290. 

Li, H. L. and Frieden, C. (2007) Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and F-19 NMR. Proceedings of the National Academy of Sciences of the U. S. A., 104(29), 11993-11998. 

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